环境与职业医学2013，Vol.30Issue(8)：611-613,3.
上海市88例乙型肝炎患者乙肝病毒基因型与基因突变的关系
Relationship between Genotypes and Mutation of Hepatitis B Virus in 88 Hepatitis B Patients in Shanghai
摘要
Abstract
[Objective] To understand the distribution of genotypes and mutation of hepatitis B virus (HBV) in hepatitis B patients.[Methods] Eighty-eight hepatitis B patients in Shanghai were randomly sampled in 2010.Nested polymerase chain reaction (PCR) was caRried out to detect genotypes of HBV in blood samples of the selected patients.The pre-S/S and C gene regions of HBV DNA were amplified and sequenced.[Results] Among the 88 subjects,52 patients (59.09％) were infected by HBV genotype C and 36 patients (40.91％) by HBV genotype B.The mean age of HBV genotype B group [(38.56 ± 12.55) years old] was lower than that of HBV genotype C group [(44.54 ± 15.72) years old] with significantly statistical difference (P＜0.05).The positive rate of hepatitis B e antigen (HBeAg) in the HBV genotype B group (66.67％) was significantly higher than that of the HBV genotype C group (28.85％) (P＜0.05).The mutation rates of C1762 and C1764 situs in HBV genotype C group were 50％ and 50.77％ respectively,statistically higher than those of the genotype B group which were 27.78％ and 25％ respectively (P＜0.05).[Conclusion] The hepatitis B patients in this study are dominantly infected with genotype C.The mutation rAtes of C1762 and C1764 situs in patients with genotype C were higher than those with genotype B.Strengthened surveillance on genotype and mutation of HBV is required.关键词
乙肝病毒/基因型/基因突变Key words
hepatitis B virus/genotype/gene mutation分类
医药卫生引用本文复制引用
黎健,陈健,李燕婷,胡家瑜..上海市88例乙型肝炎患者乙肝病毒基因型与基因突变的关系[J].环境与职业医学,2013,30(8):611-613,3.基金项目
上海市卫生局科研课题(编号:20114072) （编号:20114072）
上海市公共卫生重点学科建设计划项目(编号:12GWZX0101) （编号:12GWZX0101）
