老年医学与保健2013，Vol.19Issue(4)：239-242,4.DOI:10.3969/j.issn.1008-8296.2013-14
益智防呆方对Aβ1-40诱导大鼠海马神经元凋亡相关基因表达的影响
Effect of Yizhi-Fangdai decoction on the expression of Aβ1-40-induced apoptosis-related genes in rat hippocampal-neural cells
摘要
Abstract
Objective To investigate effect of Yizhi-Fangdai Decoction on the expression of Aβ 1-40-induced apoptosisrelated genes in rat hippocampus neural cells.Methods In this study,six animal groups were used,including blank control,model control,groups receiving different concentrations (mild,moderate and high) of herbs and Donepezil group.Experimental AD model was established by intraventricular injection of Aβ1-40.Total RNA of hippocampal neuron was extracted 4 weeks after treatment administration.Expression of Caspase-3,Bcl-2,Bax and p53 was evaluated using RT-PCR.Results Compared to blank control group,expression of Bcl-2 significantly declined,while Caspase-3,Bax and p53 significantly increased in experimental groups (P＜0.01).Treatment with Yizhi-Fangdai decoction upregulated Bcl-2 level in hippocampal neural cells (P ＜ 0.01),and down-regulated Caspase-3,Bax and p53 levels (P＜0.01).Conclusions Elevated Bcl-2 expression and inhibited Caspase-3,Bax and p53 expression with Yizhi-Fangdai decoction administration could be a possible mechanism that Yizhi-Fangdai decoction improves learning and memory function.关键词
阿尔茨海默病/凋亡/海马/神经元/益智防呆方Key words
Alzheimer's disease/ Apoptosis/ Hippocampus/ Neural cell/ Yizhi-Fangdai decoction分类
医药卫生引用本文复制引用
万文斌,赵明星,董振华,余忠海,陈久林,郁志华,李亚明..益智防呆方对Aβ1-40诱导大鼠海马神经元凋亡相关基因表达的影响[J].老年医学与保健,2013,19(4):239-242,4.基金项目
上海市科委中药现代化专项基金项目(12401904500),上海市卫生局中医药科研基金课题项目(2010J001A) （12401904500）
