泸州医学院学报Issue(4)：364-367,4.
不同产地大麻的ITS全序列分析
Identification of cannabis from different growing areas based on rDNA ITS sequencing
摘要
Abstract
objective:To identify cannabis sativa plants from Yunnan and Xinjiang areas on molecular level by internal transcribed spaces (ITS) sequence analysis. Methods:The complete ITS sequencing of cannabis sativa was determined through DNA extraction by Plant Genomic DNA Kit, PCR amplification, glue recovery and sequencing. Then the phylogenetic tree of cannabinaceae was constructed by comparing with the complete ITS sequencing of three cannabis plants from GenBank. Results: The gene sequence of cannabis by homology analysis showed that the genetic relationship between NCBI database and Xinjiang cannabis (drugs hemp) was the nearest, the homology reaches 99%; but the relationship between Yunnan cannabis (industrial hemp) and Xinjiang cannabis is farther. Conclusion: The cannabis from different sources could be identified by ITS sequence .关键词
毒品大麻/工业大麻/ITS全序列/系统发育树Key words
Marijuana hemp/Industrial hemp/Internal transcribed spaces/Phylogenetic tree分类
社会科学引用本文复制引用
代勇,姜黎,皮建华,孙琴..不同产地大麻的ITS全序列分析[J].泸州医学院学报,2012,(4):364-367,4.基金项目
四川省教育厅科研项目(09ZC053),泸州市科技局重点项目(2010-S-35) （09ZC053）
