棉花学报2013，Vol.25Issue(4)：291-299,9.
棉花精氨酸脱羧酶基因GhADC1克隆与表达分析
Cloning and Expression Analysis of an Arginine Decarboxylase Gene from Gossypium hirsutum
摘要
Abstract
Based on a sequence in a suppression subtractive hybridization library,GhADC1,GenBank accession no.KC851856,an arginine decarboxylase gene was cloned from upland cotton using bioinformatics and RT-PCR technologies.The full-length cDNA of GhADC1 was 2954-bp long.The 5' UTR was 477-bp and contained several c/s-acting elements involved in stress responses and a uORF encoding an 8-amino acid sequence.The main ORF was 2181-bp long,and encoded 726 amino acids with a putative N-terminal chloroplast-targeting signal peptide.Conserved domain analysis showed that GhADC1 contained a PLP binding site and an Orn/Dap/Arg decarboxylase signal site.These characteristics indicated that it belongs to the type Ⅲ PLP-dependent arginine decarboxylase protein family.Quantitative reverse transcription-polymerase chain reaction results showed that,under untreated conditions,the expression level of GhADC1 was higher in leaves than that in stems and roots,respectively,and was higher in the leaves of the resistant cotton variety Jiman 20 than that in those of the tolerant cotton variety Zhong 521.Furthermore,the expression of GhADC1 was rapidly up-regulated in the roots of the resistant variety by infection with Verticillium dahliae,while the tolerant variety did not show remarkable changes in its expression after infection.This indicated that the gene probably involved in the early defense responses to V.dahliae in the roots of the resistant cotton variety.关键词
棉花/精氨酸脱羧酶基因/多胺/黄萎病菌/基因表达Key words
Gossypium hirsutum/GhADC1/polyamine/Verticillium dahliae/gene expression分类
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李亚栋,默辉娟,王省芬,孙艳香,马峙英..棉花精氨酸脱羧酶基因GhADC1克隆与表达分析[J].棉花学报,2013,25(4):291-299,9.基金项目
973前期研究专项(2011CB111609) （2011CB111609）
