棉花学报2013，Vol.25Issue(4)：300-308,9.
利用高通量测序技术鉴定棉纤维发育相关miRNAs及其靶基因
Identification of Cotton microRNAs and Their Targets by High-Throughput Sequencing
摘要
Abstract
miRNAs (microRNAs) are 21-24 nt non-protein-coding small RNAs that play important roles in controlling plant growth and development by directing target mRNA cleavage or translational inhibition.In this study,we constructed libraries using ovules collected at 0 DPA (days post anthesis) and 3 DPA,and fibers collected at 10 DPA,from two interspecific backcross inbred lines (BILs).The libraries were sequenced and analyzed.There were significant differences in fiber length between the two lines,while other yield and fiber quality characters were similar.By comparison with the D5 cotton genome and other known cotton sequences,we identified 561 miRNAs,including 254 conserved miRNAs,75 candidate conserved miRNAs,and 232 new miRNAs in the libraries.These data represent a large increase in the number of known cotton miRNAs.Most miRNAs act as negative regulators of their targets,and very few miRNAs are positive regulators.Analyses of target gene functions indicated that plant hormone metabolism was the main target for miRNAs.KEGG (Kyoto encyclopedia of genes and genomes) analysis indicates that miRNA target genes were significant enriched in plant hormone pathways.关键词
棉花miRNA/高通量测序/棉花D5基因组/靶基因预测Key words
cotton miRNAs/deep sequencing/cotton D5 genome/target prediction分类
农业科技引用本文复制引用
南文智,吴嫚,于霁雯,范术丽,黄双领,李兴丽,张红卫,张金发,喻树迅..利用高通量测序技术鉴定棉纤维发育相关miRNAs及其靶基因[J].棉花学报,2013,25(4):300-308,9.基金项目
国家973计划项目(2010CB126006) （2010CB126006）
棉花生物学国家重点实验室基金(CB2013C08) （CB2013C08）
