棉花学报2013，Vol.25Issue(4)：323-328,6.
雷蒙德氏棉3条染色体特异BAC的筛选与定位
Screening and Positioning of Three Chromosome-specific BAC Clones in Gossypium raimondii
摘要
Abstract
We screened a bacterial artificial chromosome(BAC) library of Gossypium barbadense acc.Pima 90-53 to identify chromosome-specific BAC clones.Using BAC-fluorescence in situ hybridization technology,we obtained three BAC clones specific to chromosome D501,D502,and D510 of Gossypium raimondii,which could be used as cytological markers for those three chromosomes.Comparative mapping of these three BACs between G.barbadense and in G.raimondii showed that these three BAC clones could also be used to identify chromosomes Db01,Db02,and Db10 in G.barbadense.The position of the BAC clone 280G06 on chromosome 10 did not show colinearity between G.barbadense and G.raimondii,possibly because of chromosomal rearrangement.关键词
雷蒙德氏棉/单染色体/细菌人工染色体/荧光原位杂交Key words
G.raimondii/individual chromosome/bacterial artificial chromosome(BAC)/Fluorescence in situ hybridization (FISH)分类
农业科技引用本文复制引用
覃琴,刘方,甘仪梅,王春英,王玉红,蔡小彦,王坤波..雷蒙德氏棉3条染色体特异BAC的筛选与定位[J].棉花学报,2013,25(4):323-328,6.基金项目
国家863计划(2012AA101108-02-05) （2012AA101108-02-05）
农业部保种项目(NB2012-2130135-29B) （NB2012-2130135-29B）
农业部948项目(2011-G1-04) （2011-G1-04）
