棉花学报2013，Vol.25Issue(4)：345-351,7.
棉花航天诱变芽黄突变体蛋白组学分析
Proteomic Analysis of a Cotton Virescent Mutant Obtained by Space Mutation
摘要
Abstract
We extracted total protein from the second-to-top leaves from the cotton line CCRI 58 and its virescent mutant CCRI58vsp,which was obtained by space mutation.The protein profiles of the two lines were obtained by isoelectric focusing followed by SDS-PAGE.We analyzed the relative abundance of differentially expressed proteins in leaves between CCRI 58 and CCRI 58vsp using ImageMaster-2D Elite 7.0 software,and further identified the differentially expressed protein spots by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF) analysis.The results showed that 41 proteins showed differential expressions between CCRI 58 and CCRI 58vsp.The PI values of these proteins were mainly concentrated in the range of 4.0 to 7.0 and their molecular weights ranged from 15.0 to 95.0 kDa.After further MALDI-TOF/TOF analysis,we identified 14 of the differentially expression proteins.Among these were ribulose-1,5 bisphosphate carboxylase/oxygenase,S-adenosylmethionine synthase,and flavanone3-hydroxylase.The positively identified proteins were associated with photosynthesis and light respiration,ethylene and polyamine synthesis,and flavonoid synthesis.Based on these differentially expressed proteins,we can explain some of the mechanisms of the cotton virescent mutant at the protein level.关键词
棉花/芽黄突变体/蛋白质组/质谱分析Key words
cotton/virescent mutant/proteome/mass spectrum analysis分类
农业科技引用本文复制引用
李海晶,蒋博,范术丽,庞朝友,宋明梅,宋美珍,喻树迅..棉花航天诱变芽黄突变体蛋白组学分析[J].棉花学报,2013,25(4):345-351,7.基金项目
国家高技术研究发展计划(2011AA10A102)和国家棉花产业技术体系(CARS-18) （2011AA10A102）
