四川中医2013，Vol.31Issue(8)：37-40,4.
基因芯片技术研究虚热证大鼠阴虚内热的分子机制
Research on Molecular Mechanism of “Yin Asthenia with Internal Heat” in Asthenia Hot Syndrome Rats by Gene Chip Technology
摘要
Abstract
To explain molecular mechanism of “Yin Asthenia with Internal Heat” in asthenia hot syndrome rats by analysis the whole gene expression profile of the hver in rats with asthenia hot syndrome.Methods:The Asthenia hot syndrome rats models were induced by compound preparation of Traditional Chinese Medicine of Aconitum carmichaeli,Cinnamomi Cortex,Rhizoma Zingiberis.The liver gene expression in each group was detected by gene chip.We selected the differential expression genes and conducted the significant analysis on the genetic function of differential genes.A part of genes were selected to test the accuracy of results by RT-PCR.Results:As compared to the control group,in asthenia hot model group there were 99 strips of differential expression gene,mainly about function of defense response and sterol metabolic process.Conclusion:The abnormal expression of genes about defense response and sterol metabolic process in asthenia hot syndrome rats is possibly related to the substance foundation of asthenia hot syndrome.关键词
基因芯片/虚热证/阴虚内热Key words
Gene chip/ Asthenia hot syndrome/ Yin asthenia with internal heat分类
医药卫生引用本文复制引用
韩冰冰,王世军..基因芯片技术研究虚热证大鼠阴虚内热的分子机制[J].四川中医,2013,31(8):37-40,4.基金项目
国家重点基础研究发展计划(973计划)(编号:2007CB512601,2013CB531803) （973计划）
