山东农业科学2013，Vol.45Issue(8)：9-14,6.
花生CEM1基因的克隆及表达载体构建
Clone and Expression Vector Construction of Peanut Gene CEM1
摘要
Abstract
A cloned peanut sequence,named CEM1 with the GenBank accession number as EY396043,was obtained from the cDNA library of seeds and pericarp at different pod developing stages by sequencing and bioinformatics analysis.Sequencing analysis showed the CEM1 gene belonged to MADS-box family.The full length of CEM1 was 1 061 bp containing an open reading frame (ORF) as 762 bp.Its encoded protein with 253 amino acids had the molecular weight as 29.405 ku and pI as 9.18.The analysis of protein hydrophobicity and signal peptide showed that there was small possibility to cut the signal peptide and it had hydrophilcity.The VIGS expression vector of CEM1 gene was constructed,which provided a foundation for further research of its gene function.关键词
花生/荚果发育/CEM1基因/生物信息学/载体构建Key words
Peanut/Pod development/CEM1 gene/Bioinformatics/Construction of expression vector分类
生物科学引用本文复制引用
张浩,庄伟建,张廷婷,王春玮,尹红,闫彩霞,郑奕雄,单世华..花生CEM1基因的克隆及表达载体构建[J].山东农业科学,2013,45(8):9-14,6.基金项目
山东省花生产业技术体系项目 （）
山东省良种工程项目"花生高产、优质、多抗基因资源发掘与种质创新利用研究" （）
