实验动物与比较医学2013，Vol.33Issue(4)：271-274,4.DOI:10.3969/j.issn.1674-5817.2013.04.005
巴马小型猪不同发情周期的阴道细胞学观察
Vaginal Cytologic Examination on Estrous Cycle in Mini-pig
摘要
Abstract
Objective To examine the vaginal cytology of estrous cycle in Guangxi Bama mini-pig.Methods Twelve mini-pig were selected and divided into different groups-diestrus group,proestrus group and estrus group.Vaginal smears of the pigs were obtained and stained with hematologic stain and Papanicolaou stain respectively.Epithelial cell morphology was observed and counted under the optical microscope.Results Leukocytes,para basal cell,small intermediate cell,large intermediate cell and superficial cell were found in vaginal smears.Leukocyte percentage was significantly higher during diestrus than that during estrus or proestrus (P＜0.01).Para basal cell plus small intermediate cell counts were significantly higher during proestrus than that during estrus or diestrus (P＜0.01).Superficial plus large intermediate cell counts were significantly higher during estrus than that during diestrus or proestrus (P＜0.01).Conclusion Vaginal smears can be used to estimate different stage of estrous cycle in Guangxi Bama mini-pig.关键词
阴道涂片/发情周期/小型猪Key words
Vaginal smear/ Estrous cycle/ Mini-pig分类
生物科学引用本文复制引用
田芳,夏敏杰,王玉柱,周新初,李卫华,丁训诚..巴马小型猪不同发情周期的阴道细胞学观察[J].实验动物与比较医学,2013,33(4):271-274,4.基金项目
国家科技重大专项(2012ZX10001-007-009) （2012ZX10001-007-009）
十二五国家科技支撑项目(2012BAI31B04) （2012BAI31B04）
国家自然科学基金青年基金项目(81100459) （81100459）
上海市科委项目(11PJ1408400,11140902900) （11PJ1408400,11140902900）
