实验动物与比较医学2013，Vol.33Issue(4)：301-305,5.DOI:10.3969/j.issn.1674-5817.2013.04.011
裸鼹鼠与C57BL/6小鼠自噬调节的比较研究
Comparative Study on Autophagy Regulation between Naked Mole Rat and C57BL/6 Mouse
摘要
Abstract
Objective A comparative study between naked mole rat and C57BL/6 mouse on autophagy regulation is conducted,providing a reference for the research of mechanism in high autophagy,anti-cancer,slow aging of NMR.Methods Electron microscopy and western blotting are use to detect the activtity of autophagy in the liver and lung tissue of newborn naked mole rat and C57BL/6.Reagents interfere the autophagy level of fibroblasts derived from naked mole rat and mouse.The cell number is tested after 24,48,72 hours by CCK-8 kit.Meanwhile,expression of Beclin 1 at each time point is detected by western blotting.Results Compared with mice,the naked mole rat tissue have significantly higher LC3B expression.The NMR cells have a certain amount of resistance from 3-MA.Rapamycin's proliferation inhibition on NMR-S(fibroblasts derived from skin of naked mole rats)was significantly greater than that of mouse cells.Conclution NMR tissue possess a high autophagy,Cells from NMR have greater ability of self-regulation under extraneous autophagy inhibition,compared to C57BL/6.关键词
裸鼹鼠/C57BL/6小鼠/自噬调节/比较研究Key words
Naked mole rat/ C57BL/6 mouse/ Autophagy regulation/ Comparative study分类
生物科学引用本文复制引用
林丽芳,赵懿宁,赵善民,汤球,孙伟,张璐,袁子彦,徐晨,崔淑芳..裸鼹鼠与C57BL/6小鼠自噬调节的比较研究[J].实验动物与比较医学,2013,33(4):301-305,5.基金项目
上海市科技发展基金编号(11140901700及12140900200) （11140901700及12140900200）
