色谱2013，Vol.31Issue(8)：781-785,5.DOI:10.3724/SP.J.1123.2012.12051
超高效液相色谱-串联四极杆质谱法分析婴儿乳粉中的唾液酸
Analysis of sialic acid in infant formulas using ultra performance liquid chromatography-triple quadrupole mass Spectrometry
摘要
Abstract
The method for analysing sialic acid in infant formulas by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been established.Sialic acid in milk was released via acid hydrolysis,and purified by an HLB solid phase extraction cartridge.The UPLC separation was performed on an ACQUITY UPLCTM BEH HILIC column (50mm x 2.1 mm,1.7 μm) utilizing a gradient elution program of acetonitrile and water (containing 0.1％ formic acid) as the mobile phases at a flow rate of 0.25 mL/min.Injection volume and column temperature were set at 5 μL and 30 ℃,respectively.The identification and quantification were achieved by using electrospray ionisation (ESI)-MS/MS in positive ion mode and multiple reaction monitoring (MRM) mode.The linear range was from 0.05 to 5.0 mg/L for sialic acid and the correlation coefficient (R2) was greater than 0.99.The average recoveries spiked at the four concentration levels of 0.1,0.5,2.5 and 5.0 mg/L ranged between 84.3％and 98.9％ with the relative standard deviations from 4.9％ to 8.2％.The limit of detection was 0.01 mg/L.Therefore,this method has the characteristics of simple operation,high reproducibility and sensitivity.It can be widely applied to determine the total contents of sialic acid in infant formula,cow milk and human milk.关键词
超高效液相色谱-串联质谱/唾液酸/婴儿配方乳Key words
ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) / sialic acid/ infant formula分类
化学化工引用本文复制引用
解鸿蕾,李春,刘宁..超高效液相色谱-串联四极杆质谱法分析婴儿乳粉中的唾液酸[J].色谱,2013,31(8):781-785,5.基金项目
"十二五"国家科技支撑课题(2011BAD09B0304). （2011BAD09B0304）
