生物骨科材料与临床研究2013，Vol.10Issue(4)：1-7,后插1-后插2,9.DOI:10.3969/j.issn.1672-5972.2013.04.001
大鼠股骨缺损模型中BBP增强BMP-2的骨诱导作用
Enhancement of BMP binding peptide combined with recombinant human BMP-2 on the heal of a rodent segmental femoral defect
摘要
Abstract
Objective To elucidate the potential enhanced efficacy of BBP using critical-sized segmental femoral bone defects in rats.Method 70 defects in 7 groups of rats were filled with various amounts (0,2,5,and 10μg) of rhBMP-2 with or without 1000μg BBP.Radiographs were obtained after 4 and 8 weeks.The animals were euthanized at 8 weeks,and femoral specimens were assessed manually,evaluated for bone volume using microcomputed tomography,and subjected to histological or biomechanical analysis.Result Although 10μg rhBMP-2 yielded consistent results in terms of bone healing and quality of bone repair across the segmental defect,lower doses ofrhBMP-2 failed to induce satisfactory bone healing.However,the combined administration of lower doses ofrhBMP-2 and BBP induced the formation of significantly large amounts of bone.Conclusion Our results suggest that the combined administration of rhBMP-2 and BBP facilitate bone healing and have potential clinical applications.关键词
重组人骨形态发生蛋白-2/骨形态发生蛋白结合肽/股骨缺损/骨愈合Key words
rhBMP-2/ BBP/ Femur defect/Bone fusion分类
医药卫生引用本文复制引用
费志强,熊建义,刘志勇,黄江鸿,沈慧勇,刘照华,王大平,黄德民,梁加利..大鼠股骨缺损模型中BBP增强BMP-2的骨诱导作用[J].生物骨科材料与临床研究,2013,10(4):1-7,后插1-后插2,9.基金项目
中国博士后基金面上资助项目(2011M501365) （2011M501365）
广东省自然科学基金面上项目(S2012010008129) （S2012010008129）
深圳市重点实验室提升项目(ZDSY20120614154551201) （ZDSY20120614154551201）
广东省医学科研基金(项目编号B2013351) （项目编号B2013351）
深港创新圈项目(JSE201007190007) （JSE201007190007）
国家留学基金委员会公派留学资助 （）
深圳市科技创新委员会重点实验室建设项目(ZDSY20130402140954343) （ZDSY20130402140954343）
