生物信息学2013，Vol.11Issue(2)：136-141,6.DOI:10.3969/j.issn.1672-5565.2013.02.11
应用PPIN分析肝移植临床耐受患者PBMC基因表达特点
Apply protein-protein interaction network to analyze characteristics of the gene expression in PBMC of operational tolerant liver transplant recipients
摘要
Abstract
In this paper we use methods of bioinformatics to explore the characteristics of the gene expression and screen key genes in PBMCs of operational tolerant patients receiving liver transplantation using bio-informatics.A data-set of gene expression profiles of 19 operational tolerant samples of liver transplantation and 22 non-tolerant was downloaded from database GEO.We applied DAVID network software to annotate and functionally classified the differential genes.The MiMI plug-in for cytoscape was used to screen key genes related to operational tolerance by protein-protein interaction network(PPIN)analysis.Differential genes were involved in complicated and diversified biological processes such as metabolism of protein and RNA,immune response,membrane organization and so on.10 core genes highly related to operational tolerance were obtained by PPIN analysis.Our results indicate that the immune mechanism of operational tolerance of liver grafts involves sophisticated regulation mechanism of gene expression and extensive protein-protein interaction in PBMCs; RNA post-transcriptional processing and proteins degradation are important participants in regulation of operational tolerance; Core genes such as RBM8A,DHX9,CBL,IKBKB,HSPA8 etc.,play key regulatory roles in immune functions.关键词
肝移植/临床耐受/基因表达谱/蛋白质相互作用网络Key words
Liver Transplantation/ Operational Tolerance/ Gene Expression Profile/ PPIN分类
医药卫生引用本文复制引用
徐洪来,肖敏,杨超,高义,雒真龙,周鸿敏,陈忠华..应用PPIN分析肝移植临床耐受患者PBMC基因表达特点[J].生物信息学,2013,11(2):136-141,6.基金项目
国家重点基础研究发展计划(973计划)(2009CB522407). （973计划）
