陕西师范大学学报（自然科学版）2013，Vol.41Issue(3)：45-49,5.
枯草芽孢杆菌2-3-2的抗线虫作用和产酶条件与酶特性
Effect of nematode resistance and enzyme production condition and enzyme characteristics of Bacillus subtilis 2-3-2
摘要
Abstract
In order to study the relationship between nematode resistance effect of Bacillus subtilis 2-3-2 and chitinase activity,and to optimize chitinase producing condition and discuss characteristics of chitinase,pot incubation test was used to determine nematode resistance effect and the DNS method was used to determine chitinase activity.The experiment results indicated that Bacillus subtilis 2-3-2 could control Meloidogyne incognita efficiently,and the capacity of nematode resistance is related to chitinase activity.The ingredient of enzyme producing medium is as follow(g/L):colloidalchitin 65,yeast extract 1,glucose 5,KH2 PO4 0.7,K2 HPO4 0.5,MgSO4 · 7H2O 0.5,FeSO4 0.01,NaC1 0.5,microelement salt 1mg/L.After a 72-hour incubation with initial pH 7.0 at 30 ℃,the enzyme activity reaches to 35.17 × 10-10 mol/s,which is 1.59 times higher compared with the activity before optimization.The optimum chitinase producing temperature and pH of Bacillus subtilis 2-3-2 are pH 7.0 and 40℃ respectively.In an alkaline condition,if the temperature is above 40 ℃,the enzyme activity is lowered.关键词
枯草芽孢杆菌/抗线虫作用/几丁质酶/产酶条件/酶特性Key words
Bacillus subtilis/nematode-resistance/chitinase/condition of chitinase producing/properties of chitinase分类
生物科学引用本文复制引用
陶树兴,张娟妮,郭贤,柴霞,魏园媛,冯晓磊..枯草芽孢杆菌2-3-2的抗线虫作用和产酶条件与酶特性[J].陕西师范大学学报（自然科学版）,2013,41(3):45-49,5.基金项目
国家自然科学基金资助项目(10474061) （10474061）
