实用妇产科杂志2013，Vol.29Issue(6)：437-440,4.
盆腔器官脱垂患者盆底结缔组织中Fibulin-5和LOXL1的表达及意义
Expression of Fibulin-5 and Lysyl Oxidase-Like 1 in Uterosacral Ligaments and Anterior Vaginal Wall of Women with Pelvic Organ Prolapse
摘要
Abstract
Objective:To investigate the expressions of Fibulin-5 and lysyl oxidase-like 1 (LOXL1) in uterosacral ligaments and anterior vaginal wall in women with pelvic organ prolapse (POP) and their relationship with the occurrence of POP.Methods:Quantitative real-time polymerase chain reaction (real-time RT-PCR) was performed to measure the level of Fibulin-5 and LOXL1 mRNA in the uterosacral ligaments and anterior vaginal wall of 24 POP patients(POP group) and 22 patients who received hysterectomy for benign gynecological diseases (control group).Simultaneously,Fibulin-5 and LOXL1 protein expressions were assessed by immunohistochemistry.Results:①Comparing to controls,significant decrease in Fibulin-5 mRNA expression and increase of LOXL1 mRNA expression were found in the uterosacral ligaments and anterior vaginal wall of patients with POP (P＜0.05).In patients with POP Ⅱ,Ⅲ stage,the level of Fibulin-5 in the uterosacral ligaments was decreased with increasing degrees of prolapse,while the level of LOXL1 was increased with increasing degrees of prolapse (P ＜ 0.05).②2-Fibulin-5 was almost expressed in the extracellular matrix and LOXL1 in the cytoplasm of fibroblasts and smooth muscle cells.Comparing with controls,the positive rate of Fibulin-5 expression in the uterosacral ligaments and anterior vaginal wall was significantly lower in patients with POP while the positive rate of LOXL1 was significantly higher (P＜0.05).Conclusions:There is a decreased expression of Fibulin-5 and an increased expression of LOXL1 in the uterosacral ligaments and anterior vaginal wall of patients with POP.Alterations of Fibulin-5 and LOXL1 expression might impact the synthesis of elastic fibers and hence induce POP.关键词
Fibulin-5/类赖氨酰氧化酶1/盆腔器官脱垂/弹性纤维Key words
Fibulin-5/Lysyl oxidase-like 1/Pelvic organ prolapse/Elastic fiber分类
医药卫生引用本文复制引用
张晶,黄向华,张琳,赵金钗,王振海..盆腔器官脱垂患者盆底结缔组织中Fibulin-5和LOXL1的表达及意义[J].实用妇产科杂志,2013,29(6):437-440,4.基金项目
河北省医学适用技术跟踪项目(编号:CL 200811) （编号:CL 200811）
