实用肝脏病杂志2013，Vol.16Issue(3)：251-253,3.DOI:10.3969/j.issn.1672-5069.2013.03.019
阿奇霉素诱导的急性肝损伤小鼠肝组织HSP27表达的动态变化
Hepatic dynamic changes of HSP27 in mice with azithromycin-induced acute liver injury
摘要
Abstract
Objective To investigate the hepatic dynamic changes of heat shock protein 27 (HSP27) in acute liver injury induced by azithromycin in mice.Methods A mouse model of acute liver injury was induced by injection intraperitoneally of azithromycin at dose of 800mg.kg-1.The mice were sacrificed at 3h,6h,12h,24h,30h,36h,42h,48h and 54h after injection (each for 10 mice).Blood and livers from models and control animals were collected after sacrifice.Serum ALT and AST levels were determined and HSP27 in the liver tissues was detected by Western blotting.Results Serum AST and ALT activity increased progressively after azithromycin injection,peaked at 24h,and then gradually returned to the control level at 54h;HSP27 protein in the liver tissues increased significantly at 3h,peaked at 6h,started to decrease at 12h and returned to the control level at 30h.Conclusion The dynamic changes in HSP27 protein in the liver may be involved in the pathphysiological process in mice with azithromycin-induced acute liver injury.关键词
急性肝损伤/热休克蛋白27/阿奇霉素/小鼠Key words
Acute liver injury/ Heat shock protein 27 / Azithromycin/ Mice引用本文复制引用
石岩,李三强,徐正顺,马曌,乔伟强..阿奇霉素诱导的急性肝损伤小鼠肝组织HSP27表达的动态变化[J].实用肝脏病杂志,2013,16(3):251-253,3.基金项目
国家自然科学基金项目(编号:U1204802) （编号:U1204802）
河南省重点科技攻关项目(编号:122102310030) （编号:122102310030）
