实用医学杂志2013，Vol.29Issue(9)：1385-1387,3.DOI:10.3969/j.issn.1006-5725.2013.09.004
正常人外周血Th17细胞的频率、表型及其功能特征
Frequency, phenotype and functional characterization of peripheral blood Th17 cells in healthy individual
摘要
Abstract
Objective To analyze the frequency,phenotype and functional characterization of peripheral blood Th17 cells in healthy individuals.Methods PBMCs were isolated from normal health human peripheral blood and cultured with PMA and ionomycin in the presence of Brefeldin A for 4 ～ 5 h at 37 ℃ in a 5％ CO2humidified atmosphere.The frequency of subpopulation of IL-17-producing cells,the expression of IL-17 with Th1 and Th2 cytokines and the phenotypic characterization of IL-17-producing cells were assessed by flow cytometry.Results The frequency of Th17 cells was (1.63 ± 0.72)％ following stimulation with PMA plus ionomycin.Th17 cells express CD45RO,CCR6 and CCR4.About fifteen percentage of Th17 cells showed a double IL-17+ IFN-γ+ phenotype,more than eighty percentage of Th17 cells showed a double IL-17+IL-2+phenotype,whereas cells producing both IL-17 and Th2 cytokines were almost undetectable.Conclusion Memory Th17 cells with the expression of CCR6 and CCR4 are apparent in healthy individual PBMCs and Th17 cells are different from Th1/Th2 cells.关键词
流式细胞术/Th17细胞/表型/细胞因子Key words
Flow cytometry/T helper 17 cell/Phenotype/Cytokine引用本文复制引用
周茂华,张敏,欧阳维富,吴璐思..正常人外周血Th17细胞的频率、表型及其功能特征[J].实用医学杂志,2013,29(9):1385-1387,3.基金项目
广东省医学科研基金课题(编号:A2009042) （编号:A2009042）
