实用医学杂志2013，Vol.29Issue(9)：1406-1408,3.DOI:10.3969/j.issn.1006-5725.2013.09.011
骨髓单个核细胞移植在脑梗死大鼠脑内分化为小胶质样细胞的实验研究
Study on the transdifferentiation of bone marrow mononuclear cells into microglia transplanted in the brains of cerebral infarction rats
摘要
Abstract
Objective To investigate the therapeutic effect and differentiation of bone marrow mononuclear cells (BMMNCs) transplanted in the brains of cerebral infarction rats.Methods The middle cerebral artery occlusion models (pMCAO) were established by intraluminal vascular suture method,the successful models were randomly divided into the vehicle group (vehicle group) and BMMNCs transplantation group (BMMNCs group),BMMNCs group was injected 1 mL cell suspension containing 1 × 107 BMMNCs marked by Brdu via the tail vein after 24 h,vehicle group was injected the same amount of PBS.Neurological severity scores (Nss) was used to observe neural behavioral function,double immunofluorescent labeling was utilized to trace the differentiation of BMMNCs in the rats' brains.Results Compared with vehicle group,Nss of BMMNCs group was significantly lower (P ＜ 0.05),Brdu and Ibal double-labeled positive cells were found in the infarcted cortex and hippocampal dentate gyms in BMMNCs group.Conclusion BMMNCs transplanted in the brains of cerebral infarction rats can differentiate into microglia,and improve the neural behavioral function of rats following cerebral infarction.关键词
脑梗死/骨髓单个核细胞/移植/小胶质细胞/分化Key words
Brain infarction/Bone marrow mononuclear cells/Transplantation/Microglia/Differentiation引用本文复制引用
王建平,陈明,蒋超,余列,王梦涵,刘恒方,张敏,赵源征,陈宁宁..骨髓单个核细胞移植在脑梗死大鼠脑内分化为小胶质样细胞的实验研究[J].实用医学杂志,2013,29(9):1406-1408,3.基金项目
国家自然科学基金资助项目(编号:81271284) （编号:81271284）
