实用医学杂志2013，Vol.29Issue(10)：1549-1552,4.DOI:10.3969/j.issn.1006-5725.2013.10.002
rHSG基因对COPD大鼠气道成纤维细胞增殖凋亡的调控
Regulation of rHSG on the proliferation and apoptosis of airway fibroblasts of rats with chronic obstructive pulmonary disease
摘要
Abstract
Objective To study the effects of exogenous rat hyperplasia suppressor gene (rHSG) on the proliferation of airway fibroblasts of rats with COPD.Methods The recombinant plasmid pEGFP-rHSG was transfected into airway fibroblasts of rats with COPD,then the expressions of rHSG mRNA at 24 h,48 h,3 d,5 d,7 d after transfection were detected by real-time fluorescence quantitative polymerase chain reaction (real time-PCR),and the proliferation and apoptosis of the transfected airway fibroblasts were measured by MTT and flow cytometry.Results The expression of rHSG mRNA began to increase at 24 h after transfection,and reached peak at 7 d.The proliferation of airway fibroblasts was significantly inhibited in rHSG transfection group than that in model control group at 48 h after transfection (P ＜ 0.05),while the apoptosis rate of airway fibroblasts was increased at 48 h,and reached peak at 7 d after transfection (P ＜ 0.05).Conclusion Exogenous rHSG can suppress the proliferation and induce apoptosis of airway fibroblast.关键词
肺疾病,慢性阻塞性/气道重塑/气道成纤维细胞/大鼠增殖抑制基因Key words
Chronic obstructive pulmonary disease (COPD)/Airway remodeling/Airway Fibroblast/rHSG gene引用本文复制引用
葛正行,李波,周洵,李常..rHSG基因对COPD大鼠气道成纤维细胞增殖凋亡的调控[J].实用医学杂志,2013,29(10):1549-1552,4.基金项目
国家自然基金地区科学基金资助项目(编号:81060319) （编号:81060319）
