土壤学报2013，Vol.50Issue(4)：810-817,8.DOI:10.11766/trxb201208010308
一株拮抗放线菌的鉴定及其对黄瓜枯萎病的生防效应研究
IDENTIFICATION AND BIOCONTROL EFFECT OF A STRAIN OF ACTINOMYCETE ANTAGONISTIC TO WILT DISEASE OF CUCUMBER
摘要
Abstract
Morphological,cultural and physio-biochemical traits and 16S rRNA sequence was analyzed of a strain of actinomycete,separated from the soil of the Zijin Mountain in Nanjing,China and coded as CT205,for identification and orientation in the classification system.The PDA agar plate method was used to determine antifungal activity of Strain CT205 and a pot experiment using pathogenic soil from a field that had been cultivated with cucumber consecutively for year was conducted to investigate the effect of Stain CT205 controlling cucumber Fusarium wilt.Based on the 16S rRNA sequence analysis,Strain CT205 was tentatively identified as Streptomyces albospinus (99％ identity).And the pot experiment showed that the strain inhibited quite effectively Fusarium oxysporum f.sp.cucumerinum,Fusarium oxysporum f.sp.niveum,and Phytophthora nicotianae,and to a varying extent,Bacillus subtillus,Saccharomyces cerevisiae,Rhizoctonia cerealis,Ralstonia solanacearum.Besides its cucumber Fusarium wilt controlling efficiency being 51.85％,the strain promoted somewhat the growth of cucumber.When the strain was prepared with organic manure into complex biomanure,the disease controlling efficiency may reach as high as 81.85％.The findings demonstrate that the strain has certain potential application value.关键词
放线菌CT205/鉴定/黄瓜枯萎病/土壤微生物/生物防治Key words
Antagonistic actinomyces Strain CT205/ Identification/ Cucumber wilt disease/ Soil microorganism/Biological control分类
农业科技引用本文复制引用
梁银,张谷月,王辰,王世梅,沈其荣..一株拮抗放线菌的鉴定及其对黄瓜枯萎病的生防效应研究[J].土壤学报,2013,50(4):810-817,8.基金项目
国家自然科学基金项目(40871126)资助 （40871126）
