西北师范大学学报（自然科学版）2013，Vol.49Issue(4)：76-82,100,8.
Con A型差异糖蛋白在大鼠肝再生中的作用研究
Study of the role of differential Con A glycoprotein in rat liver regeneration
摘要
Abstract
To explore the role of glycoprotein in rat liver regeneration,this study analyzes the glycoprotein differential expression profile during rat liver regeneration using Concanavalin-A affinity chromatography,2D-PAGE and MALDI-TOF/TOF technology.The result shows that,among 123 proteins,there are 117 proteins which has potential N-or O-glycosylation site.Difference analysis indicates that,33 proteins have differential expression during liver regeneration,the numbers of up-,down-and up/down-regulated proteins are 20,9 and 4,respectively.Pathway analysis by IPA software shows that,these differential proteins mainly participate in p70S6K and PI3K/AKT signaling pathway which maybe regulate liver regeneration.Further analysis reveals that,the protein of NR1H4,GNMT,F16P,BGLR,H6PD,ALDH,14-3-3Z,ASSY,CYTB and ACTB are closely related to rat liver regeneration after partial hepatectomy.This research will contribute to study the glycopeptideˊs structure of glycoprotein and the bio-function of protein glycosylation,especially the role in liver regeneration.关键词
大鼠/肝再生/Con A亲和层析/糖蛋白/双向电泳/质谱鉴定Key words
rat/regenerative liver/Con A affinity chromatography/glycoprotein/two-dimensional gel electrophoresis/mass spectrometry分类
生物科学引用本文复制引用
杨彦杰,杨军英,张亚磊,耿小芳,徐存拴..Con A型差异糖蛋白在大鼠肝再生中的作用研究[J].西北师范大学学报（自然科学版）,2013,49(4):76-82,100,8.基金项目
973前期研究专项(2012CB722304) （2012CB722304）
河南省基础研究与前沿项目(122300410253) （122300410253）
