新疆农业科学2013，Vol.50Issue(6)：981-987,7.DOI:10.6048/j.issn.1001-4330.2013.06.001
新陆早33号高效遗传转化体系研究
Study on Protocol of Efficiency Genetic Transformation in Xinluzao 33 (Gossypium hirsutum L.)
摘要
Abstract
[Objective]In order to carry out the transgenic protocol in cotton,the effect of different treatments on resistant embryogenic callus proliferation,somatic embryogenesis and regeneration of upland cotton (Gossypium hirsutum L.) Xinluzao 33 was studied.[Method] Hypocotyls of Xinluzao 33 were taken as explants,and glyphosate-resistant gene EPSPS was transformed by Agrobacterium-mediated transformation method.Somatic embryogenesis capacity and cotyledonary embryos culture methods were optimized.[Result]During the period of embryogenic callus proliferation,when the concentration of the culture medium was 3.8g/L KNO3,11 days subculture cycle were suitable for fast proliferation and keeping better state.Phytagel in MSB medium supplemented with 4.0 g/L obviously promoted embryoid differentiation,and with 1.0 g/L activated carbon facilitated root development,reduced browning and improved the ratio of normal regenerated plant.[Conclusion] The cycle of regeneration via somatic embryogenesis was shortened and a large number of regenerated plants were obtained.The efficient genetic transformation protocol for Xinluzao 33 was constructed.关键词
棉花/抗性胚性愈伤组织/体细胞胚胎发生/再生植株Key words
cotton/resistant embryogenic callus/somatic embryogenesis/regenerated plants分类
农业科技引用本文复制引用
李鹏飞,朱华国,程文翰,王凡龙,张新宇,孙杰..新陆早33号高效遗传转化体系研究[J].新疆农业科学,2013,50(6):981-987,7.基金项目
农业部转基因生物新品种培育重大专项(2011ZX08005) （2011ZX08005）
"十二五"科技支撑计划项目(2011BAD35B05) （2011BAD35B05）
石河子大学动植物育种专项(gxjs2010-yz01) （gxjs2010-yz01）
