新疆农业科学2013，Vol.50Issue(7)：1192-1198,7.DOI:10.6048/j.issn.1001-4330.2013.07.003
玉米ZmPsaH基因真核表达载体的构建及转化
Construction and Transformation of Eukaryotic Express Vector of ZmPsaH Gene from Maize
摘要
Abstract
[Objective] The psaH gene is one subunit of photosystem Ⅰ,and it plays an important role in Photosynthesis.The purpose of this paper is to provide the basis for the study of ZmpsaH gene on resistance.[Method] Construction of ZmPsaH eukaryotic expression vector is an important way to understand the gene function in maize.The ZmPsaH gene was cloned from the maize by RT-PCR,which was named ZmPsaH (photosystem Ⅰ reaction center subunit H).It was digested by the restriction enzymes SalⅠ and NotⅠ,and then connected with pREP-5N to obtain the recombinant eukaryotic expression plasmid ZmPsaH-5N.[Result]Identified by colony PCR,restriction enzyme digestion and sequencing methods,the results showed that the recombinant eukaryotic expression the plasmid ZmPsaH-5N was successfully constructed.Preparation of yeast competent,electric shock,the recombinant plasmid was transformed into yeast.A yeast drop-PCR result showed that electroporation was successful.[Conclusion] Construction of ZmPsaH gene eukaryotic expression vector and transformation are successful.关键词
玉米/ZmPsaH/真核表达载体/重组质粒构建Key words
maize/ ZmpsaH/ eukaryotic expression vector/ construction of recombinant plasmid分类
农业科技引用本文复制引用
刘小利,郝晓燕,陈勋基,陈果,足木热木,邵琳,黄全生..玉米ZmPsaH基因真核表达载体的构建及转化[J].新疆农业科学,2013,50(7):1192-1198,7.基金项目
新疆高技术研究发展计划(201011109) （201011109）
