新疆农业科学2013，Vol.50Issue(7)：1277-1287,11.DOI:10.6048/j.issn.1001-4330.2013.07.015
一株番茄枯萎病生防菌的鉴定、定殖与盆栽防效研究
Identification, Colonization and Control Effect of an Antagonistic Bacterium against Fusarium wilt Tomato
摘要
Abstract
[Objective] To understand classification,colonization ability and control effect of strain S-13,which was isolated from the field soil planted for years.[Method] Phylogenetic analysis of 16S rDNA was conducted to identify the strain.A mutant strain of S-13 which could withstand 300 μg/mL rifampicin was tested for its control effect,colonization ability and influence on rhizosphere soil microflora by pot experiment and DGGE analysis.[Result] S-13 strain was identified as Bacillus amyloliquefaciens (GU323369.1)approximate species.Pot results show that:irrigating group and spraying group of the mutant strain of S-13 have obvious control effect on tomato wilt within 45 days compared with control effects up to 100％ and 93％ respectively.Isolation and culture results show that the mutant strain of S-13 could colonize in rhizosphere soil,roots,stems,leaves of tomato after inoculation of 7,25 and 45 days,and the colonization levels in soil and root are relatively higher than other positions up to 1.6 × 105 cfu/g rhizosphere soil and 6.6 × 104 cfu/g fresh root weight at the 45th day,while only 4 × 102 cfu/g fresh leaf weight was detected in leaves of irrigating group to show that the colonization is not stable in the stems and leaves.The mutant strain of S-13 was tested by DGGE analysis to be the dominant bacteria in the soil which reduce the diversity of soil bacteria in 25 days,but afterwards the influence of the strain declined and a stable bacteria community formed with antagonistic bacterium at the 45th day.[Conclusion] Studies have shown that the antagonistic bacterium of S-13 could colonize in rhizosphere soil,roots,stems,leaves of tomato with the highest colonization level in soil and root.The tested strain has obvious effect of inhibiting the occurrence and spread of Fusarium wilt.关键词
番茄枯萎病/拮抗菌/定殖/生物防治Key words
Fusarium wilt tomato/ antagonistic bacteria/ colonization/ biological control分类
农业科技引用本文复制引用
詹发强,侯敏,杨蓉,龙宣杞..一株番茄枯萎病生防菌的鉴定、定殖与盆栽防效研究[J].新疆农业科学,2013,50(7):1277-1287,11.基金项目
新疆维吾尔自治区科技计划项目"设施农业多价菌剂的研制"(201010103) （201010103）
新疆乌鲁木齐市研究计划项目"设施农业复合菌剂的研制"(Y111210003) （Y111210003）
