新疆农业科学2013，Vol.50Issue(7)：1288-1296,9.DOI:10.6048/j.issn.1001-4330.2013.07.016
新疆冰川冰缘冻土低温淀粉酶部菌株分子生物学鉴定及酶学特性研究
Molecular Biologic Identification of Some Bacteria Strains Producing Low Temperature Amylase Isolated and Enzymatic Characterization from Frozen Soil in Xinjiang Glacier Ice Margin
摘要
Abstract
[Objective] In this paper,some bacterial strains producing low temperature amylase isolated from frozen soil in Xinjiang periglacial zone were identified by molecular biology method and analysis of its enzymatic characterization.[Method] Genome DNA was extracted from bacterial strains activated in seed nutrient solution,followed by PCR amplification and the product was compared by NCBI data base,the 16S rDNA of bacteria strains producing low temperature-amylase was analyzed by MegAlign Fasta,using improved Yoo method to measure low temperature amylase enzyme.[Result]The result showed that similarity rules of the sequences between A-2,A-3,A-4 bacterial strains analyzed by 16S rDNA and the Bacillus amyloliquefaciens database were up to 99％.This strain produced the optimum temperature amylase is 30℃,and the optimum pH is 6.[Conclusion] The conclusion was that bacteria strains of A-2,A-3,A-4 were all Bacillus amyloliquefaciens.关键词
冻土/低温淀粉酶/解淀粉芽孢杆菌/16S rDNA/分子鉴定/酶学特性Key words
Frozen soil/ low temperature amylase/ Bacillus amyloliquefaciens/ 16s rDNA/ Molecular identification / Enzyme characteristics分类
生物科学引用本文复制引用
武运,库米拉,张晓燕,王伟,古丽娜孜,王璐,陈卫林,苗森,高雯君..新疆冰川冰缘冻土低温淀粉酶部菌株分子生物学鉴定及酶学特性研究[J].新疆农业科学,2013,50(7):1288-1296,9.基金项目
新疆维吾尔自治区科技支疆项目(20101230750) （20101230750）
