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RP-HPLC法测定多花柽柳中没食子酸含量

廖菁 孙宇 卿德刚 张娟 倪慧 贾晓光

新疆农业科学2013,Vol.50Issue(7):1310-1313,4.
新疆农业科学2013,Vol.50Issue(7):1310-1313,4.DOI:10.6048/j.issn.1001-4330.2013.07.019

RP-HPLC法测定多花柽柳中没食子酸含量

Determination of Gallic acid in Tamarix hohenackeri Bunge by HPLC

廖菁 1孙宇 2卿德刚 2张娟 2倪慧 2贾晓光2

作者信息

  • 1. 新疆大学生命科学与技术学院,乌鲁木齐830046
  • 2. 新疆堆吾尔自治区中药民族药研究所,乌鲁木齐830002
  • 折叠

摘要

Abstract

[Objective] The purpose of this study was to establish a method for determining the Gallic acid in Tamarix hohenackeri Bunge.[Method]The assay was performed on a Waters XTerra RP C18(250 ×4.6 mm,5 μm) column by a isocratic elution program with acetonitrile-0.5% acetic acid as mobile phase at a flow rate of 1.0 mL/min,and the column temperature was 25℃.[Result]The linear ranges of gallic acid was 0.139-1.390 μg,and the average recoveries (n =6) were 100.59% (RSD =1.25 %).[Conclusion] This method is sample,accurate highly sensitive and reproducible.It could be effective in measuring the content of gallic acid in Tamarix hohenackeri Bunge,provides theoretical basis and methods for the quality control of Tamarix chinensis medicinal materials.

关键词

多花柽柳/没食子酸/含量测定/HPLC

Key words

Tamarix hohenackeri Bunge/ Gallic acid/ Determination/ HPLC

分类

轻工纺织

引用本文复制引用

廖菁,孙宇,卿德刚,张娟,倪慧,贾晓光..RP-HPLC法测定多花柽柳中没食子酸含量[J].新疆农业科学,2013,50(7):1310-1313,4.

基金项目

国际科技合作项目(2010DFB30540) (2010DFB30540)

新疆农业科学

OA北大核心CSCDCSTPCD

1001-4330

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