新疆农业科学2013，Vol.50Issue(7)：1330-1339,10.DOI:10.6048/j.issn.1001-4330.2013.07.022
策勒黑羊BMPR-IB、MTNR1A和PTGS2基因的RFLP分析
RFLP Analysis of BMPR-IB, MTNR1A and PTGS2 Gene in Cele Black Sheep
摘要
Abstract
[Objective] Looking for DNA mark about sheep prolificacy trait,it is to provide the theoretical basis for the development of sheep fertility marker assisted selection (MAS).[Method] In this experiment,the polymorphism of BMPR-IB,MTNR1A and PTGS2 gene in Cele black sheep was detected by PCR-RFLP and DNA sequencing.[Result]The results showed,that BMPR-IB gene in Cele black sheep had polymorphism,with three kinds of genotype:+ +,B + and BB; MTNR1A and PTGS2 gene didnt have polymorphism.[Conclusion]The fact that mutational site of A746G of BMPR-IB gene is prolificacy major gene in Cele black sheep is checked,not MTNR1A and PTGS2 gene.关键词
策勒黑羊/多胎性状/BMPR-IB/MTNR1A/PTGS2Key words
Cele black sheep/ prolificacy trait/ BMPR-IB/ MTNR1A/ PTGS2分类
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王琼,邵勇钢,刘玲玲,田佳,古丽尼沙·吐拉甫,努孜古丽·图尔荪,耿明,莫合塔尔·夏甫开提,刘武军..策勒黑羊BMPR-IB、MTNR1A和PTGS2基因的RFLP分析[J].新疆农业科学,2013,50(7):1330-1339,10.基金项目
新疆维吾尔自治区高技术研究发展项目"新疆绵羊品种主要经济性状功能基因的鉴定"(201111115) （201111115）
