新疆农业科学2013，Vol.50Issue(7)：1347-1352,6.DOI:10.6048/j.issn.1001-4330.2013.07.024
多浪羊IL-1β基因的克隆及序列分析
Cloning and Sequence Analysis of IL-1β from Duolang Sheep in Xinjiang
摘要
Abstract
[Objective]The purpose of this project aims to clone and analyse the IL-1β gene in order to lay a foundation for genetic screening of Duolang sheep.[Method] The study was based on the primers from genebank and the total RNA was extracted from Duolang sheep lymphocytes,and then the IL-1 βby was amplified by RT-PCR.After sequencing the IL-1β,the gene contained an ORF of 801 bp,and the length of amino acid mature protein coding.[Result] Four relative conserved bases were found at the position of 70,163,180,269 which mutations occurred by multiple sequence alignment; the two amino acid mutations occurred at the position of 24 and 36 after multiple amino acid alignment; as a result,we know that the homology between Duolang sheep and other ordinary sheep is up to 99％.[Conclusion] The evolution reviews that Duolang sheep and the other mammals are originated from the same ancestor,but located at the different branches of the evolutionary tree.关键词
多浪羊/IL-1β/基因克隆/核苷酸序列分析Key words
Duolang sheep/ IL-1 β/ gene cloning/ nucleotide analysis分类
农业科技引用本文复制引用
曾国航,曹玉华,王娟娟,潘伊微,贾山玲,徐宏伟,李莲瑞..多浪羊IL-1β基因的克隆及序列分析[J].新疆农业科学,2013,50(7):1347-1352,6.基金项目
国家自然科学基金"棉酚对新疆多浪羊白细胞介素表达影响研究"(30960277) （30960277）
