畜牧兽医学报2013，Vol.44Issue(6)：858-865,8.DOI:10.11843/j.issn.0366-6964.2013.06.005
FSH和17β-雌二醇联合作用对Cyclin D1 mRNA和Cyclin E1 mRNA表达的影响
Effect of Combination of FSH and 17β-estradiol on the mRNA Expression of Cyclin D1 and Cyclin E1 in the Cultured Immature Boar Sertoli Cell
摘要
Abstract
The objective of the study was to identify whether the combination of FSH and 17-betaestradiol regulated the mRNA expression of Cyclin D1 and Cyclin E1 in the cultured immature boar Sertoli cells.Cultured immature boar Sertoli cells were treated with the combination of 17-beta-estradiol (10-9 mol · L-1) and FSH (50 ng · mL-1) and added a variety of different signaling pathway inhibitors.And Real-time PCR was applied to detect the mRNA expression of Cyclin D1 and Cyclin E1.Compared to FSH (50 ng · mL-1) or 17-beta-estradio1 (10-9 mol · L-1)alone,the combination of FSH and 17-beta-estradiol had no significant effect on the mRNA expression of Cyclin D1 mRNA and Cyclin E1 (P＞0.05).In addition,Rp-cAMP (cAMP inhibitor),Verapamil (L-type Ca2+ ionic channel inhibitor) or U0126 (ERK1/2 inhibitor) alone had no significant effect on the mRNA expression of Cyclin D1 and Cyclin E1 in comparison to the control group (no FSH or17-beta-estradiol) (P＞0.05).However,three inhibitors could reduce the mRNA expression of Cyclin D1 and Cyclin E1 in a dose-dependent way (P＜0.05,for all) when compared to the combined FSH (50 ng · mL-1) and 17-beta-estradiol (10-9 mol · L-1).cAMP,Ca2+ and ERK1/2 were involved in the effect of the combination of FSH and 17-beta-estradiol regulating the mRNA expression of Cyclin D1 and Cyclin E1.关键词
FSH/17β-雌二醇/睾丸支持细胞/细胞周期蛋白D1/细胞周期蛋白E1/细胞外信号调节的蛋白激酶1/2Key words
FSH /17-beta-estradiol/Sertoli cell/Cyclin D1 /Cyclin E1/extracellular signal-regulated kinase 1/2分类
农业科技引用本文复制引用
王怡,张姣姣,汪勇,张会琼,孙思,王鲜忠,张家骅..FSH和17β-雌二醇联合作用对Cyclin D1 mRNA和Cyclin E1 mRNA表达的影响[J].畜牧兽医学报,2013,44(6):858-865,8.基金项目
国家自然科学基金项目(31072183) （31072183）
