畜牧兽医学报2013，Vol.44Issue(6)：911-918,8.DOI:10.11843/j.issn.0366-6964.2013.06.012
基于纳米材料的电化学免疫传感器检测HSN1亚型禽流感病毒的研究
A Electrochemical Immunosensor Detection of Avian Influenza Virus (H5N1) Based on Nano Materials
摘要
Abstract
A novel electrochemical immunosensor for sensitive detection of avian influenza virus (AIV) H5N1 was described.A graphene oxide (GO) carrying H5N1-polychonal antibodies (PAb-H5N1) and Bovine serum albumin (BSA) were used as signal amplification materials.On the basis of the signal amplification strategy of PAb-H5N1-GO-BSA nanocomposite,the developed immunosensor showed a 256-fold increase in detection limit compared to the immunosensor without PAb-H5N1-GO-BSA nanocomposite amplification.The developed method could detect 2-15 HA unit · 50 μL-1 H5N1 with a linear calibration range from 2-15 to 2-8 HA unit · 50 μL-1.This immunosensor has a good specificity and high sensitivity,and it is promising for rapid detection of pathogenic microorganisms.关键词
氧化石墨烯/电化学免疫传感器/金纳米粒子/H5N1亚型禽流感病毒Key words
graphene oxide/electrochemical immunoassay/gold nanoparticles/avian influenza virus H5N1分类
农业科技引用本文复制引用
黄娇玲,谢芝勋,罗思思,谢志勤,谢丽基,刘加波,庞耀珊,范晴..基于纳米材料的电化学免疫传感器检测HSN1亚型禽流感病毒的研究[J].畜牧兽医学报,2013,44(6):911-918,8.基金项目
广西特聘专家专项经费(2011B020) （2011B020）
广西科技攻关重大专项(1222003-2-4) （1222003-2-4）
