畜牧兽医学报2013，Vol.44Issue(6)：965-971,7.DOI:10.11843/j.issn.0366-6964.2013.06.020
四甲基秋兰姆二硫化物对肉鸡胫骨生长板超微结构的影响
The Ultrastructure Changes of Tibia Growth Plate in Broiler Chickens with a Thiram-feeding Procedure
摘要
Abstract
The aim of this study was to study the ultrastructure of tibia growth plate in broiler chickens with a thiram (Tetramethylthiuram disulfide)-feeding procedure at the different stages.Seven-day-old AA broiler chickens were fed a regular diet containing thiram 100 mg · kg-1 for 48h,then were dissected quickly,and growth plates were fixed in 2.5％ glutaric dialdehyde solution in 4 ℃ when they were 8,9,13 day-old.The ultrastructure of chondrocyte was observed by transmission electron microscope.The results showed that proliferative chondrocytes were slightly necrotic,increased numbers of swollen mitochondrion appeared electron-dense particles and vesiculated cisternae of the endoplasmic reticulum were in rounding at 8 day-old,and swollen mitochondrion,breaked and dilated cisternae of the endoplasmic reticulum were more severe at 9 dayold; plasmolysis and doubtful apoptotic prehypertrophic chondrocytes were discovered at 8,9day-old,moreover,lighten necrotic prehypertrophic chondrocytes with complete cellular structure and increased secretory vacuoles with fine particles of proteoglycans emerged at 13 day-old.In conclusion,both proliferative cells and prehypertrophic chondrocytes of growth plates were damaged by thiram in a short time,especialy to prehypertrophic chondrocytes.We supposed that this changes would influence normal chondrocyte development.It was possible that thiram could disturb the energy metabolism and cellular signal transduction which confused the cell cycle,and resulted in tibial dischondroplasia.关键词
福美双/肉鸡胫骨生长板/超微结构/软骨细胞Key words
thiram/broiler tibia growth plate/ultrastructure/chondrocyte分类
农业科技引用本文复制引用
田文霞,李家奎,王瑞,覃平,宁官保,乔建钢,李宏全,毕丁仁,潘思轶..四甲基秋兰姆二硫化物对肉鸡胫骨生长板超微结构的影响[J].畜牧兽医学报,2013,44(6):965-971,7.基金项目
中国博士后科学基金(20080430984) （20080430984）
国家自然科学基金(30571370,31072179) （30571370,31072179）
山西省自然科学基金(2009011043-1) （2009011043-1）
山西农业大学博士科研启动基金. （）
