畜牧与兽医2013，Vol.45Issue(7)：26-29,4.
牛瑟氏泰勒虫二温式PCR快速检测技术的建立及初步应用
Establishment of two-temperature PCR for the rapid detection of Theileria sergenti and its preliminary application
摘要
Abstract
To establish a rapid method to detect Theileria sergenti,a pair of primers was designed according to the gene encoding P23 major surface protein of T.sergenti(D84447).The 244 bp gene fragment was amplified,and the sequencing analysis showed 100％ of homology with known gene sequence.The two-temperature PCR was established and its highest susceptibility was 171 fg/μL.The method showed no cross reaction with bovine Neospora caninum,Toxoplasma gondii and Babesia ovata.In 48 tested clinical blood samples,the positive rate was 66.67％.When 28 clinical blood samples were detected using the same pair of primers by two-temperature and three-temperature PCR,respectively,the positive coincidence rate was 95％,and the whole coincidence rate was 96.43％.The results indicated that the two-temperature PCR could be used in the rapid clinical diagnosis and epidemiological investigation in T.sergenti infection.关键词
牛瑟氏泰勒虫/P23基因/二温式PCRKey words
Theileria sergenti/P23 gene/two-temperature PCR分类
农业科技引用本文复制引用
丁晓双,夏晓辉,张晓轩,海旭楠,许应天..牛瑟氏泰勒虫二温式PCR快速检测技术的建立及初步应用[J].畜牧与兽医,2013,45(7):26-29,4.基金项目
延边大学“211工程”三期重点建设项目 （）
