医疗卫生装备2013，Vol.34Issue(8)：71-72,75,3.DOI:10.7687/J.ISSN.1003-8868.2013.08.071
紫外双光束扫描检测血清中的百草枯
Serum Paraquat Detection by UV Double-beam Spectrophotometer
摘要
Abstract
Objective To apply UV spectrophotometer for the diagnosis of paraquat poisoning and develop a method for qualitative and quantitative detection of serum paraquat by UV spectrophotometer.Methods The serum had its protein precipitated with 20％ TCA,and then went through 10-min storage in the-20 ℃ refrigerator and 12 000 r/min high-speed centrifugation.The superuatant was measured by UV spectrophotometer,with 50 μL microcell used.Results Linear correlation occurred when the serum paraquat concentration was limited between 0.10 μg/mL and 40 μg/mL,with the regression equation of y=0.081 2x+0.0104,the coefficient correlation of 0.999 6,the recovery rate between 92.5％ and 103.0％,relative standard deviation between(RSD) 3.6％ and 4.7％.The RSD was between 2.8％ and 4.8％ in a day and between 2.2％ and 4.6％ in several days,with minimum detection limit of 0.05 μg/mL.Conclusion A simple and accurate method is provided for the qualitative and quantitative detection of serum paraquat of the poisoning patient,which can be used for paraquat concentration measuring before and after hemoperfusion.[Chinese Medical Equipment Journal,关键词
血清/百草枯/紫外分光光度法Key words
serum/ paraquat/ UV spectrophotometry分类
医药卫生引用本文复制引用
陈姿如,陈礼明,杜书明..紫外双光束扫描检测血清中的百草枯[J].医疗卫生装备,2013,34(8):71-72,75,3.
