亚热带植物科学Issue(3)：25-28,4.
‘石牌’广藿香叶肉原生质体的分离及纯化
Isolation and Purification of Mesophyll Protoplasts of Pogostem cablin cv. shipaiensis
摘要
Abstract
Mesophyll protoplasts were isolated and purified from leaves of in vitro propagated Polgostemon cablin (Blanco) Benth. cv. shipaiensis, and different factors affecting protoplasts yield and activity were investigated. The results showed that young leaves of seedling subcultured for 12~22 days in the enzyme solution consisting of 0.5% (w/v) pectolyase Y-23, 0.2%(w/v) Macerozyme R-10 and 1. 5% (w/v) cellulase R-10, in 11% (w/v) mannitol buffered with 0.1% MES and 0.02%CaCl2 for 8 h, the yield was 1.85×107 protoplasts per gram leaves (fresh weight), the viability was above 89%.关键词
‘石牌’广藿香/原生质体分离/叶肉原生质体Key words
Pogostem cablin cv. shipaiensis/protoplast isolation/mesophyll protoplast分类
农业科技引用本文复制引用
莫小路,邱蔚芬,黄珊珊,陈瑜珍,严振..‘石牌’广藿香叶肉原生质体的分离及纯化[J].亚热带植物科学,2012,(3):25-28,4.基金项目
广东省科技厅粤港关键领域重点突破项目(2009A030901011)、广州市科技计划基础研究项目(2009J1-C201) （2009A030901011）
