应用生态学报2013，Vol.24Issue(8)：2281-2286,6.
不同香蕉枯萎病区土壤细菌群落多样性
Diversity of soil bacterial community in banana orchards infected with wilt disease
摘要
Abstract
Six soil samples including 3 wilt disease-infected samples and 3 disease-free samples were collected from the banana orchards in 3 areas in Lingao County,Hainan Province of South China.The soil physical and chemical properties were determined by conventional methods,and the diversity of soil bacterial community was analyzed by terminal restriction fragment length polymorphism (T-RFLP).Then,the relationships between the soil bacterial community composition and the soil physical and chemical properties were investigated.In the same areas,most of the soil physical and chemical properties were poorer in disease-infected than in disease-free banana orchards,with the most obvious difference in soil available P content and pH.The T-RFLP analysis showed the diversity of soil bacterial community was richer in disease-infected than in disease-free banana orchards.The lengths of the dominant T-RFs in the 3 areas were 144,147 and 233 bp,respectively.Through the comparison with phylogenetic assignment tool,it was deduced that the dominant species in the 3 areas were Bacillus subtilis,Staphylococcus and Eubacterium ruminantium.The distribution of most T-RFs was related to the soil alkaline hydrolyzable N,available K,available P and water content,and the relative abundance of most T-RFs was richer in disease-infected than in disease-free banana orchards.关键词
香蕉枯萎病/土壤因子/细菌群落多样性/T-RFLPKey words
banana's wilt disease/soil factor/bacterial community diversity/T-RFLP分类
农业科技引用本文复制引用
陈波,黄霄,刘小玉,周登博,谭昕,高祝芬,张锡炎,戚春林..不同香蕉枯萎病区土壤细菌群落多样性[J].应用生态学报,2013,24(8):2281-2286,6.基金项目
现代农业产业技术体系建设专项资金项目(CARS-32)资助. （CARS-32）
