应用生态学报2013，Vol.24Issue(8)：2294-2300,7.
石灰性土壤拉恩式溶磷细菌的筛选鉴定及溶磷特性
Screening, identification and phosphate-solubilizing characteristics of Rahnella sp.phosphate-solubilizing bacteria in calcareous soil
摘要
Abstract
Several strains of phosphate-solubilizing bacteria were isolated and screened from the crop rhizosphere of calcareous soil in Shanxi Province of China.After repeated isolation and purification,the strain W25 with strong phosphate-solubilizing activity was obtained,and identified as Rahnella sp.,based on the morphological,physiological and biochemical properties and the analysis of 16S rRNA gene sequence.Further studies on the W25 showed that the maximum phosphatesolubilizing capability of the W25 on tricalium phosphate,aluminum phosphate and ferric phosphate reached 385.5,110.4 and 216.6 mg · L-1,respectively.In the liquid culture with aluminum phosphate and ferric phosphate,the solubilized phosphorous by the W25 was significantly negatively correlated with the liquid pH,with the correlation coefficient being 0.56 and 0.81,respectively.Among the carbon and nitrogen sources,glucose and ammonium nitrate were the optimum for the solubilization of tricalium phosphate by W25.The utilization of carbon source was in the order of glucose ＞ lactose ＞ sucrose ＞ mannitose ＞ starch,and that of nitrogen source was in the order of ammonium nitrate ＞ ammonium chloride ＞ ammonium sulfate ＞ potassium nitrate ＞ sodium nitrate.Different nitrogen sources had greater effects on the production of organic acids by W25.Formic acid and acetic acid would be produced when the nitrogen source was NH4+,oxalic acid and succinic acid would be produced when the nitrogen source was NO3-,and citric acid would be extra produced when the ammonium nitrate was used as the nitrogen source.关键词
石灰性土壤/拉恩式菌/溶磷特性Key words
calcareous soil/Rahnella sp./phosphate-solubilizing characteristics分类
农业科技引用本文复制引用
乔志伟,洪坚平,谢英荷,李林轩..石灰性土壤拉恩式溶磷细菌的筛选鉴定及溶磷特性[J].应用生态学报,2013,24(8):2294-2300,7.基金项目
国家公益性行业项目(201103004-5)和山西省研究生创新项目(20123045)资助. （201103004-5）
