中国病理生理杂志2013，Vol.29Issue(7)：1186-1191,6.DOI:10.3969/j.issn.1000-4718.2013.07.007
SNS-032诱导弥漫性大B淋巴瘤OCI-LY-19细胞株凋亡及其作用机制的研究
Mechanism of SNS-032-induced apoptosis of human diffuse large B-cell lymphoma cell line OCI-LY-19
摘要
Abstract
AIM:To investigate the effects of SNS-032 on the apoptosis of human diffuse large B-cell lymphoma cell line OCI-LY-19 and its underlying mechanisms.METHODS:OCI-LY-19 cells were treated with different concentrations of SNS-032.The cell viability was measured by MTS assay.The cell death was analyzed by propidium iodide (PI) staining,and the cel apoptosis was analyzed by flow cytometry with Annexin V/PI staining.The expression of apoptosis-re-lated and prliferation-related proteins was analyzed by Western blotting.RESULTS:The viability and proliferation of OCI-LY-19 cells was inhibited by SNS-032 and the IC50 was 0.358 μmol/L.The percentage of apoptotie cells was increased by SNS-032 in a dose-and time-dependent manner.Poly(ADP-ribose) polymerase (PARP) protein was cleaved after SNS-032 treatment.The expression of apoptosis-related proteins,procaspase-3,procaspase-9,X-linked inhibitor of apoptosis protein (XIAP) and myeloid cell leukemia-1 (Mcl-1),and proliferation-related proteins,protein serine/threonine kinase (Akt),phosphory-lated Akt (p-Akt),signal transductor and activator of transcription 5 (STAT5),phosphorylted STAT5 (p-STAT5) and phosphorylated extracellular signal-regulated kinase (p-ERK),was down-regulated by SNS-032 in a dose-and time-dependent manner.Cleaved caspase-3 and cleaved caspase-9 expression levels were devated after SNS-032 treatment.The expression of total ERK was not obviously changed.CONCLUSION:SNS-032 can induce apoptosis of OCI-LY-19 cells and the mechanism may associate with the cleavage of PARP,the down-regulation of apoptosis-related proteins and the inhibition of proliferation-related JAK/STAT,MEK/ERK and PI3K/Akt signaling pathways.关键词
SNS-032/弥漫性大B淋巴瘤/细胞凋亡Key words
SNS-032/ Diffuse large B-cell lymphoma/ Apoptosis分类
医药卫生引用本文复制引用
师宪平,蓝晓莹,温创宇,陈鑫,刘焕亮,黄美近..SNS-032诱导弥漫性大B淋巴瘤OCI-LY-19细胞株凋亡及其作用机制的研究[J].中国病理生理杂志,2013,29(7):1186-1191,6.基金项目
国家自然科学基金资助项目(No.81100378) （No.81100378）
广州市科信局应用基础研究专项重点项目(No.2012J4100014) （No.2012J4100014）
广东省医学科研基金资助项目(No.B2012159) （No.B2012159）
