中国病理生理杂志2013，Vol.29Issue(7)：1192-1196,5.DOI:10.3969/j.issn.1000-4718.2013.07.008
4E-BP1在人脑胶质瘤细胞对卡氮芥获得性耐药中的作用
4E-BP1 regulates sensitivity of human glioma cells to carmustine
摘要
Abstract
AIM:For finding new target to overcome acquired drug resistance of glioma,the relationship between eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) and acquired drug resistance to carmustine [1,3-bis(2-chloroethyl)-1-nitrosourea,BCNU] in human glioma cells was investigated.METHODS:Western blotting was used to analyze the expression levels of 4E-BP1 and phosphorylated 4E-BP1 (p-4E-BP1) in SWOZ2 cels and BCNU-re-sistant SWOZ2 (SWOZ2-BCNU) cells.The siRNA targeting to human 4E-BP1 mRNA was transfected into SWOZ2 cells.Cell counting kit-8 was used to detect the sensitivity of the cells to BCNU before and after transfection.The location of 4E-BP1 and p-4E-BP1 (T70) in SWOZ2 cells and SWOZ2-BCNU cells was observed by the method of immunofluorescence.RESULTS:The expression of 4E-BP1 in SWOZ2-BCNU cells was significantly lower than that in SWOZ2 cells.Down-reg-ulation of 4E-BP1 expression in SWOZ2 cells decreased the sensitivity of the cells to BCNU.The expression of p-4E-BP1(T70) in the nucleus of SWOZ2-BCNU cells was significantly higher than that in the cytosol.CONCLUSION:4E-BP1 is involved in human glioma acquired drug resistance as a translation key protein.关键词
真核翻译起始因子4E结合蛋白1/神经胶质瘤/获得性耐药Key words
Eukaryotic translation initiation factor 4E-binding protein 1/ Glioma/ Acquired drug resistance分类
医药卫生引用本文复制引用
朱慧丽,翁泽平,张继君,林琛莅,谢思明,王超,马继伟,钟雪云..4E-BP1在人脑胶质瘤细胞对卡氮芥获得性耐药中的作用[J].中国病理生理杂志,2013,29(7):1192-1196,5.基金项目
国家自然科学基金资助项目(No.81072059) （No.81072059）
广东省高等学校科技创新重点项目(No.CXZD1110) （No.CXZD1110）
广东省医学科研基金资助项目(No.B2011159) （No.B2011159）
