中国病理生理杂志2013，Vol.29Issue(7)：1239-1243,5.DOI:10.3969/j.issn.1000-4718.2013.07.017
二十二碳六烯酸对大鼠肺动脉平滑肌细胞大电导钙激活性钾通道的影响
Effects of docosahexaenoic acid on large-conductance calcium-activated potassium channels in rat pulmonary artery smooth muscle cells
摘要
Abstract
AIM:To investigate the effects of docosahexaenoic acid (DHA) on large-conductance calcium-ac-tivated potassium channels (BKCa) in rat pulmonary artery smooth muscle cells (PASMCs).METHODS:BKCa currents in individual PASMCs were recorded by patch-clamp technique in whole-cell configuration.Calcium sparks in PASMCs caused by DHA were recorded by confocal microscopy.RESULTS:DHA activated BKCa.BKCa current densities were (30.5 ±6.5) pA/pF,(59.4 + 5.8) pA/pF,(87.2 + 4.3) pA/pF and (117.3 ± 7.1) pA/pF (P ＜ 0.01) with the addition of DHA at concentrations of 0,0.1,1 and 10 μmol/L,respectively.Hypoxia inhibited BKCa currents in PASMCs,but this inhibition was reversed by DHA (10 μmol/L).DHA (10 μmol/L) induced an increase in [Ca2+]i with a maximal increase rate of (71.9 +4.1)％.CONCLUSION:DHA activates BKCa in rat PASMCs,leading to the vasodilation of pulmonary arteries.关键词
肺动脉平滑肌细胞/二十二碳六烯酸/大电导钙激活钾通道/缺氧Key words
Pulmonary artery smooth muscle cells/ Docosahexaenoic acid/ Large-conductance calcium-activated potassium channels/ Hypoxia分类
医药卫生引用本文复制引用
陈蕊,刘培晶,严金川,顾雨春..二十二碳六烯酸对大鼠肺动脉平滑肌细胞大电导钙激活性钾通道的影响[J].中国病理生理杂志,2013,29(7):1239-1243,5.基金项目
国家自然科学基金资助项目(No.81170279) （No.81170279）
江苏省自然科学基金资助项目(No.BK2010335 （）
No.BK2011486) （）
江苏省科教兴卫工程(No.LJ201116) （No.LJ201116）
镇江市心血管病重点实验室(No.SS2012002) （No.SS2012002）
