中国病理生理杂志2013，Vol.29Issue(7)：1339-1344,6.DOI:10.3969/j.issn.1000-4718.2013.07.034
人类CD8+记忆T细胞体外扩增方法的研究
Methods for expansion of human CD8 + memory T cells in vitro
摘要
Abstract
AIM:To investigate new methods for the expansion of human CD8 + memory T cells in vitro and provide novel means of anti-viral and anti-tumor adoptive immunotherapy.METHODS:Six kinds of stimuli,anti-CD3 antibody,anti-CD28 antibody,CD70,IL-2,IL-7 and IL-15,for the expansion of human CD8+ memory T cells in vitro were selected and arranged for their combinations,resulting in 63 kinds of stimulating combinations.Normal human CD8 + T cells were isolated and exposed to these stimuli.After 14 days of cell culture,the number and purity of CD8 + T cells,and the percentages of CD8 + central memory T cells (TCM) and CD8 + effector memory T cells (TEM) were detected.The expansion folds of CD8 + T cells,CD8 + TCM and CD8 + TEM were calculated and the relatively better stimulating combination was determined.RESULTS:The combination of anti-CD3 antibodies,IL-2 and IL-7 was a better method for the expansion of human CD8 + T cells,CD8 + TCM and CD8 + TEM in vitro,and the expansion folds were 13.19,13.28 and 15.27,respectively.CONCLUSION:The combination of anti-CD3 antibodies,IL-2 and IL-7 is the relatively better method for the expansion of human CD8 + memory T cellsin vitro.关键词
过继性免疫治疗/CD8+记忆T细胞/体外扩增Key words
Adoptive immunotherapy/ CD8 + memory T cells/ In vitro expansion分类
医药卫生引用本文复制引用
罗海华,周南,张旭,郭学敏,张辉,刘超..人类CD8+记忆T细胞体外扩增方法的研究[J].中国病理生理杂志,2013,29(7):1339-1344,6.基金项目
"十二五"艾滋病和病毒性肝炎等重大传染病防治科技重大专项基金资助项目(No.2012ZX10001-003-004 （）
No.2013ZX10001-004) （）
国家自然科学基金资助项目(No.30972620 （）
No.81101255) （）
广东省自然科学基金资助项目(No.9251008901000022) （No.9251008901000022）
高等学校博士学科点专项科研基金资助项目(No.20090171110083 （）
No.20100171120056) （）
