中国动脉硬化杂志2013，Vol.21Issue(8)：680-684,5.
二十二碳六烯酸对低氧性肺血管收缩的影响及机制
Effects of Doeosahexecnoic Acid on Hypoxic Pulmonary Vasoconstriction and Its Mechanism
摘要
Abstract
Aim To study the effects of doeosahexecnoic acid (DHA) on hypoxic pulmonary vasoconstriction (HPV) and its mechanism.Methods Pulmonary arteries were cut by 2 to 3 mm for vascular rings.Following the vascular constriction induced by acute hypoxia,DHA was administrated and the relaxing forces were measured without endothelia.Using the whole cell patch-clamp technique in freshly isolated pulmonary artery smooth muscle cells,the effects of DHA on total K + currents,the large conductance Ca2+-activated K + (BKca) and voltage gated K+ currents were recorded.Results DHA relaxed vascular ring tone without endothelia (P ＜ 0.05).DHA could significantly activate total K + currents in pulmonary artery smooth muscle cells (P ＜ 0.01).It also greatly enhanced BKca currents (P ＜ 0.05) and at a testing potential of + 60 mV,125.21％ ± 5.62％ of BKca currents were increased.In contrast,DHA inhibited K+ currents and at a testing potential of + 60 mV,63.21％ ± 7.32％ of K+ currents were decreased.Conclusion DHA can activate BKca channels and relax the vascular constriction induced by acute hypoxia.关键词
二十二碳六烯酸/低氧性肺血管收缩/肺动脉平滑肌细胞/钾电流Key words
Doeosahexecnoic Acid/ Hypoxic Pulmonary Vasoconstriction/ Pulmonary Artery Smooth Muscle Cell/ K + Current分类
生物科学引用本文复制引用
刘培晶,陈蕊,严金川,王中群..二十二碳六烯酸对低氧性肺血管收缩的影响及机制[J].中国动脉硬化杂志,2013,21(8):680-684,5.基金项目
国家自然科学基金(81170279) （81170279）
江苏省自然科学基金(BK2010335 （）
BK2011486) （）
江苏省科教兴卫工程(LJ201116) （LJ201116）
镇江市心血管病重点实验室资助项目(SS2012002) （SS2012002）
