中国动脉硬化杂志2013，Vol.21Issue(8)：700-704,5.
Caveolin-1对血管吻合口白细胞介素10和白细胞介素6表达的影响
Effect of Caveolin-1 on the Expression of Interleukin-10 and Interleukin-6 in Vascular Anastomotic Stoma
摘要
Abstract
Aim To investigate the effect of the caveolin-1 on the expression of interleukin-10(IL-10) and interleukin-6 (IL-6) in rabbit carotid artery anastomotic stoma.Methods 32 New Zealand white rabbits were randomly divided into sham operation group,operation group,empty vector group and caveolin-1 transfected group.The rest specimens were taken for HE staining at 7 d and 28 d,which were used to observe the proliferation of intima,and the ratio of intima/media area was measured by Image-Pro Plus 6.0 software ; Real time PCR was used to detect the mRNA,and immunohistochemistry was used to assay the expression of protein.Results Anti-inflammatory cytokine IL-10 and pro-inflammatory cytokine IL-6 were increased by carotid artery end-to-end-intestinal anastomosis.Local transfected caveolin-1 plasmid can increase the expression of caveolin-1 mRNA in rabbit carotid artery anastomotic stoma.Compared with other groups,the mRNA and protein of IL-10 were increased in caveolin-1 transfected group.However,IL-6,a potent pro-inflammatory cytokine,was decreased in caveolin-1 transfected group.Conclusion Caveolin-1 promotes anti-inflammatory factor in the balance between the pro-and anti-inflammatory cytokines in rabbit carotid artery anastomotic stoma.关键词
小凹蛋白/白细胞介素6/白细胞介素10/吻合口Key words
Caveolin-1 / Interleukin 6 / Interleukin 10 / Anastomotic Stoma分类
医药卫生引用本文复制引用
熊国祚,戴先鹏,毕国善,申昕,邓礼明,颜亚平,庹勤慧..Caveolin-1对血管吻合口白细胞介素10和白细胞介素6表达的影响[J].中国动脉硬化杂志,2013,21(8):700-704,5.基金项目
湖南省教育厅基金(09C845),湖南省高校创新平台开放基金(12K096),南华大学研究生科研创新项目(2011XCX22)资助 （09C845）
