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水生动物中分离的副溶血性弧菌菌株分子分型研究

史秀杰 郑晓聪 阮周曦 贾鹏 何俊强 王津津 兰文升 杨锦舜 刘荭

中国动物检疫Issue(6):47-53,63,8.
中国动物检疫Issue(6):47-53,63,8.

水生动物中分离的副溶血性弧菌菌株分子分型研究

Molecular Subtyping Studies of Vibrio parahaemolyticus Isolated from Aquatic Animals

史秀杰 1郑晓聪 1阮周曦 1贾鹏 1何俊强 1王津津 1兰文升 1杨锦舜 1刘荭1

作者信息

  • 1. 深圳出入境检验检疫局动植物检验检疫技术中心,广东深圳 518045
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摘要

Abstract

  59 strains of Vibrio parahaemolyticus isolated from aquatic animals were subtyped by ribotyping(RT),pulsed-field gel electrophoresis(PFGE)and multilocus sequence typing(MLST). 59 strains were divided into 9 clusters including 43 ribotypes with discriminatory index 0.9754,13 clusters including 51 PFGE types with discriminatory index 0.9988,and 53 ST types by MLST including 107 new alleles and 46 new ST types with discriminatory index 0.9982. PFGE and MLST had higher discriminatory ability than RT. The results appeared that Vibrio parahaemolyticus had the highly rate of gene mutation.

关键词

副溶血性弧菌/核糖体分型/脉冲场凝胶电泳/多位点序列分型

Key words

Vibrio parahaemolyticus/Ribotyping(RT)/Pulsed-field gel electrophoresis(PFGE)/Multilocus sequence typing(MLST)

分类

农业科技

引用本文复制引用

史秀杰,郑晓聪,阮周曦,贾鹏,何俊强,王津津,兰文升,杨锦舜,刘荭..水生动物中分离的副溶血性弧菌菌株分子分型研究[J].中国动物检疫,2012,(6):47-53,63,8.

基金项目

深圳出入境检验检疫局科研项目(SZ2007007) (SZ2007007)

中国动物检疫

1005-944X

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