中国动物检疫Issue(9)：39-42,4.
猪繁殖与呼吸综合征病毒单克隆抗体的制备和鉴定
Preparation and Identification of Monoclonal Antibodies against PRRSV
摘要
Abstract
Objective To prepare and identify monoclonal antibodies (McAbs) against PRRSV. Methods BALB/c mice were immunized with the purified PRRSV,and monoclonal antibodies were obtained by means of hybridoma technique. The resultant monoclonal antibodies were identified for subtype,titer and evaluated for specificity. Result Two hybridoma cell lines stably secreting specific McAbs against M protein of PRRSV were obtained,named as 3C3 and 3D2,which were identified as immunoglobulin G2a (IgG2a)kappa chain,and the ELISA and IPMA titers of ascites were 1:105 to 1:107 and 1:2560 to 1:10240 respectively. The prepared McAbs showed specific reaction against PRRSV,and not reacted with CSFV、PRV、PPV and PCV. Conclusion Two McAbs against PRRSV have been successfully prepared,further facilitating which may the study of rapid detection techniques for PRRSV.关键词
猪繁殖与呼吸综合征病毒/单克隆抗体/酶联免疫吸附试验/免疫过氧化物酶单层试验Key words
PRRSV/Monoclonal Antibodies/ELISA/IPMA分类
农业科技引用本文复制引用
曹振,邓小雨,张倩,倪建强,周智,遇秀玲,赵德明,田克恭..猪繁殖与呼吸综合征病毒单克隆抗体的制备和鉴定[J].中国动物检疫,2012,(9):39-42,4.基金项目
“北京市生猪产业技术体系创新团队”课题(GWZJ-2009-05) （GWZJ-2009-05）
