中国动物检疫Issue(9)：49-51,52,4.
贝类派琴虫和折光马尔太虫二重 PCR 方法的建立
Development of a Duplex PCR assay for Detection of Perkinsus and Marteilia refringens in Shellfish
摘要
Abstract
Two pairs of specific primers were designed according to the conserved regions on the sequences of Perkinsus sp and Marteilia refringens in GenBank. A duplex transcription polymerase chain reaction was optimized to simultaneously detect the two pathogens,Perkinsus sp and Marteilia refringens in shellfish. It was shown that all samples containg Perkinsus sp and Marteilia refringens could be amplified into two specific bands,596bp for Perkinsus sp and 478bp for Marteilia refringens by this duplex PCR,but no specific bands of the same sizes were amplified from other shellfish pathogens such as Haplosporidium sp,Aeromonas hydrophila,Pseudomonas fluorescens,Vibrio parahaemolyticu,Vibrio Alginolyticu,Vibrio fluvialis and Vibrio mimicus. As low as 10pg of Perkinsus sp and Marteilia refringens DNA could be detected.119 Oyster samples from Guangxi coastal area were detected by this PCR. The result showed that the positive rate was 9.24% and 1.68% for Perkinsus sp and Marteilia refringens respectively, suggesting that Perkinsus sp and Marteilia refringens existed in cultivated shellfish in south China.The PCR could be used as a sensitive tool to detect Perkinsus sp and Marteilia refringens in clinical samples.关键词
派琴虫/折光马尔太虫/二重PCRKey words
Perkinsus sp/Marteilia refringens/duplex PCR分类
农业科技引用本文复制引用
谢丽基,谢芝勋,庞耀珊,刘加波,邓显文,谢志勤..贝类派琴虫和折光马尔太虫二重 PCR 方法的建立[J].中国动物检疫,2012,(9):49-51,52,4.基金项目
国家百千万人才工程人选专项资金项目(No.945200603)、广西特聘专家专项经费(2011B020)和广西科技攻关项目(桂科攻0630001-3M)共同资助 （No.945200603）
