中国动物检疫Issue(12)：26-28,3.
贝类折光马尔太虫半套式PCR检测方法的建立及初步应用
Development and Primary Application of A semi-nested Polymerase Chain Reaction assay for detection of Marteilia refringens in Shellfish
摘要
Abstract
According to the gene sequences of Marteilia refringens in GenBank,three specific primers were designed for amplifying the specific fragments of Marteilia refringens. The reaction parameters were optimized to develop the semi-nested PCR method for detection of Marteilia refringens. The specific 228 pb DNA fragment of Marteilia refringens was amplified,but not from other pathogenic such as Haplosporidium sp,Perkinsus sp,Vibrio parahaemolyticu,Vibrio alginolyticu,and Vibrio fluvialis by this semi-nested PCR. The sensitivity results showed that as little as 0.01fg DNA plasmid of Marteilia refringens was detected by this semi-nested PCR. Shellfish samples of Fujian coastal were detected by this semi-nested PCR. As a result,seven razor clam samples were positive for Marteilia refringens,suggesting that Marteilia refringens existed in the cultivated shellfish in Fujian and this semi-nested PCR could be used as a sensitive tool to detect Marteilia refringens in clinical samples.关键词
贝类/折光马尔太虫/半套式PCRKey words
Shellfish/Marteilia refringens/semi-nested PCR分类
农业科技引用本文复制引用
谢丽基,谢芝勋,刘加波,庞耀珊,邓显文,谢志勤,范晴..贝类折光马尔太虫半套式PCR检测方法的建立及初步应用[J].中国动物检疫,2012,(12):26-28,3.基金项目
国家百千万人才工程人选专项(945200603)、广西特聘专家专项(2011B020)和广西科技攻关(桂科攻0630001-3M)共同资助 （945200603）
