中国林副特产Issue(5)：1-3,3.
嗜酸乳杆菌细菌素的分离纯化及表面活性剂对其活性的影响1)
Purification of the Bacteriocin Isolated from Lactobacillus acidophilus and Effect of Surfactant on the Antibacterial Activity of Bacteriocin
摘要
Abstract
Bacteriocin which is a kind of protein with antibacterial activity is produced from L actobacillus acidophilus ,and the strain is isolated from kefir grain preserved in the lab .The separation method about bacteriocin and the influence of the different surfactants on the antibacterial activity of bacteriocin is stud -ied .The results show that L actobacillus acidophilus is cultured in MRS medium addind oleic acid after 16h culture ,a peak of the bacteriocin-producing is reached .And The purification method about bacterio-cin includes three operations ,which separately are ammonium sulfate precipitation ,ion exchange chroma-tography(IEC)and Hydrophobic Interaction Chromatography . The different kinds of surfactants may have the different effects on the antibacterial activity of bacteriocin .关键词
细菌素/嗜酸乳杆菌/表面活性剂/抑菌活性Key words
Bacteriocin/Lactobacillus acidophilus/Surfactant/Antibacterial activity引用本文复制引用
张帅,王金凤,马永强..嗜酸乳杆菌细菌素的分离纯化及表面活性剂对其活性的影响1)[J].中国林副特产,2012,(5):1-3,3.基金项目
1)黑龙江省科技厅科技攻关计划重大项目(GA07B401-2)资助 （GA07B401-2）
