中国脑血管病杂志2013，Vol.10Issue(8)：421-424,4.DOI:10.3969/j.issn.1672-5921.2013.08.006
外源性硫化氢对大鼠全脑缺血-再灌注损伤的保护作用
Protective effect of exogenous hydrogen sulfide on global cerebral ischemia-reperfusion injury in rats
任彩丽 1张国富 2蔡德亮1
作者信息
- 1. 214000,无锡同仁康复医院神经康复科
- 2. 无锡市精神卫生中心精神康复科
- 折叠
摘要
Abstract
Objective To study the protective effect and mechanisms of exogenous hydrogen sulfide (H2S,NaHS) on global cerebral ischemia reperfusion injury in rats.Methods A total of 18 healthy male SD rats were randomly allocated into sham operation,isotonic saline,and NaHS intervention groups (n =6 in each group).A rat global cerebral ischemia-reperfusion model was induced by using the modified four-vessel occlusion method.After ischemia-reperfusion in rats,the rats in the NaHS intervention group were intraperitoneally injected NaHS (25 μmol/kg) immediately,and the rats in the isotonic saline group were intraperitoneally injected isotonic saline,both once a day for 7 days.The rats in the sham operation group only expose the vessels and did not perform any other treatments.The levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in rat hippocampal tissue were detected,and thionine staining was used to observe the neuronal density of hippocampal CA1 regions.Results ①The SOD levels of the isotonic saline group (133 ± 12 kU/g) and NaHS intervention group (229 ± 21 kU/g) were lower than that (297 ±24 kU/g) of the sham operation group,and the SOD level of the isotonic saline group was lower than that in the NaHS intervention group.The differences were statistically significant (P ＜ 0.01).②The MDA levels of the isotonic saline group (55.4 ± 6.2 μmol/g) and the NaHS intervention group (22.5 ±7.9 μmol/g) were higher than that of the sham operation group (6.1 ± 1.3 μmol/g),and the MDA level of the isotonic saline group was higher than that of the NaHS intervention group.The differences were statistically significant (P ＜ 0.01).③The neuronal densities of the isotonic saline group (88 ± 15/mm2) and the NaHS intervention group (156 ± 19/mm2) were lower than that of the sham operation group (237 ±25/mm2),and the neuronal density of the isotonic saline group was lower than that of the NaHS intervention group.The differences were statistically significant (P ＜ 0.01).Conclusion H2S has protective effect for cerebral ischemia-reperfusion injury in rats.Its mechanisms may be associated with the increased antioxidase activities and anti-lipid peroxidation injury.关键词
硫化氢/脑缺血/再灌注损伤/脂质过氧化作用/大鼠Key words
Hydrogen sulfide / Brain ischemia/ Reperfusioningury/ Lipid peroxidation / Rats引用本文复制引用
任彩丽,张国富,蔡德亮..外源性硫化氢对大鼠全脑缺血-再灌注损伤的保护作用[J].中国脑血管病杂志,2013,10(8):421-424,4.
