中国人兽共患病学报2013，Vol.29Issue(7)：671-675,5.DOI:10.3969/cjz.j.issn.1002-2694.2013.07.006
福建华溪蟹线粒体DNA COI和16S rRNA基因序列的遗传多样性
Genetic diversity on mitochondrial DNA COI and 16S rRNA of Sinopotamon fukienense
摘要
Abstract
The aim of this study was to explore the genetic diversity of the freshwater crab S.fukienense based on the gene sequence CO I and 16S rRNA.The genes of CO I and 16S rRNA were amplified with PCR and subsequently sequenced.The base composition and sequence variation of them were analyed with Mega version 4.0 and DnaSP version 4.10.We obtained 639 bp nucleotide sequence of gene COI and 526 bp nucleotide sequence of gene 16S rRNA.The nucleotide diversity based on gene COI (Pi=0.048 4) was higher than that based on 16S rRNA (Pi=0.021 6).At the same time,the average genetic distance of haplotypes based on gene COI (P=0.048) was larger than that based on 16S rRNA (P=0.026).Results suggest that the COI sequence is better than the 16S rRNA sequence in the analysis of genetic mutation for S.fukienense.关键词
福建华溪蟹/COI基因序列/16S rRNA基因序列/遗传多样性Key words
S.fukienense/ CO I and 16S rRNA gene sequence/ genetic diversity分类
医药卫生引用本文复制引用
石林波,张小燕,汪雁,王云龙,周宪民,邹节新..福建华溪蟹线粒体DNA COI和16S rRNA基因序列的遗传多样性[J].中国人兽共患病学报,2013,29(7):671-675,5.基金项目
Supported by the National Natural Science Foundation of China (Nos.81060136 & 81160207),the Science Program of Jiangxi Provincial Department of Education (Nos.07078 & 12054),the Undergraduate Innovational Experimentation Program of Nanchang University (No.2008013),and the National Undergraduate Innovational Experimentation Program (No.091040308) （Nos.81060136 & 81160207）
国家自然科学基金项目(No.81060136,81160207),江西省教育厅科技计划(No.07078,12054),南昌大学大学生创新性训练计划(No.2008013)和国家大学生创新性实验计划(No.091040308)联合资助 （No.81060136,81160207）
